Celsius°
PEPTIDES
Certificate of Analysis
Starfield Processing Limited · 111 New Union Street
Coventry CV1 2NT · England
Product
GHK-Cu
Lot number
CP2606-6580
Catalogue number
CP-1009
Research area
Repair & Regenerative
Date of manufacture
2026-06-02
Date of analysis
2026-06-07
Retest by
2029-05-22
Appearance
White lyophilised solid
Purity by RP-HPLC (area normalisation, 220 nm)
99.68%
Specification ≥ 99.3%
RELEASED
9/9 parameters within specification
Analytical results
| Test | Method | Specification | Result | Status |
|---|---|---|---|---|
| Appearance | Visual inspection | White to off-white lyophilised solid | White lyophilised solid | Pass |
| Identity | ESI-MS (positive ion) | [M+H]⁺ 404.95 ± 1.0 Da | 405.12 Da | Pass |
| Purity | RP-HPLC, 220 nm, C18 gradient | ≥ 99.3 % (area normalisation) | 99.68 % | Pass |
| Single largest impurity | RP-HPLC, 220 nm | ≤ 1.00 % | 0.137 % | Pass |
| Water content | Karl Fischer titration | ≤ 8.0 % | 4.8 % | Pass |
| Acetate content | Ion chromatography | ≤ 12.0 % | 7.8 % | Pass |
| Peptide content | Amino acid analysis | ≥ 70.0 % | 88.9 % | Pass |
| Bacterial endotoxin | LAL kinetic chromogenic | ≤ 0.50 EU/mg | 0.145 EU/mg | Pass |
| Solubility | Visual, per stated solvent | Freely soluble in water; deep blue solution | Conforms | Pass |
Figure 1 · RP-HPLC chromatogram
C18, 4.6 × 250 mm, 5 µm. Water/acetonitrile gradient with 0.1% TFA over 20 minutes. Detection at 220 nm.
| Peak | Retention time | Area % | Assignment |
|---|---|---|---|
| 1 | 5.96 min | 0.137 | Acetylated analogue |
| 2 | 8.00 min | 99.680 | GHK-Cu |
| 3 | 10.58 min | 0.062 | Acetylated analogue |
| 4 | 10.84 min | 0.121 | Truncated sequence |
Figure 2 · ESI mass spectrum
Positive ion electrospray. Observed charge states with theoretical comparison below.
Molecular formula
C14H24N6O4·Cu
Theoretical [M+H]⁺
404.95 Da
Observed [M+H]⁺
405.12 Da
Sequence
Gly-His-Lys · Cu(II)
GHK