Celsius°
PEPTIDES
Certificate of Analysis
Starfield Processing Limited · 111 New Union Street
Coventry CV1 2NT · England
Product
Semax
Lot number
CP2605-8432
Catalogue number
CP-3001
Research area
Neurological & Cognitive
Date of manufacture
2026-05-03
Date of analysis
2026-05-06
Retest by
2029-04-20
Appearance
White lyophilised solid
Purity by RP-HPLC (area normalisation, 220 nm)
99.34%
Specification ≥ 99.0%
RELEASED
9/9 parameters within specification
Analytical results
| Test | Method | Specification | Result | Status |
|---|---|---|---|---|
| Appearance | Visual inspection | White to off-white lyophilised solid | White lyophilised solid | Pass |
| Identity | ESI-MS (positive ion) | [M+H]⁺ 814.93 ± 1.0 Da | 814.91 Da | Pass |
| Purity | RP-HPLC, 220 nm, C18 gradient | ≥ 99.0 % (area normalisation) | 99.34 % | Pass |
| Single largest impurity | RP-HPLC, 220 nm | ≤ 1.00 % | 0.306 % | Pass |
| Water content | Karl Fischer titration | ≤ 8.0 % | 3.6 % | Pass |
| Acetate content | Ion chromatography | ≤ 12.0 % | 8.3 % | Pass |
| Peptide content | Amino acid analysis | ≥ 70.0 % | 81.2 % | Pass |
| Bacterial endotoxin | LAL kinetic chromogenic | ≤ 0.50 EU/mg | 0.084 EU/mg | Pass |
| Solubility | Visual, per stated solvent | Freely soluble in water | Conforms | Pass |
Figure 1 · RP-HPLC chromatogram
C18, 4.6 × 250 mm, 5 µm. Water/acetonitrile gradient with 0.1% TFA over 20 minutes. Detection at 220 nm.
| Peak | Retention time | Area % | Assignment |
|---|---|---|---|
| 1 | 9.36 min | 0.077 | Truncated sequence |
| 2 | 11.09 min | 0.149 | Deletion sequence |
| 3 | 11.81 min | 99.340 | Semax |
| 4 | 13.11 min | 0.128 | Oxidised analogue |
| 5 | 13.85 min | 0.306 | Des-amido analogue |
Figure 2 · ESI mass spectrum
Positive ion electrospray. Observed charge states with theoretical comparison below.
Molecular formula
C37H51N9O10S
Theoretical [M+H]⁺
814.93 Da
Observed [M+H]⁺
814.91 Da
Sequence
Met-Glu-His-Phe-Pro-Gly-Pro
MEHFPGP