Celsius°
PEPTIDES
Certificate of Analysis
Starfield Processing Limited · 111 New Union Street
Coventry CV1 2NT · England
Product
Semax
Lot number
CP2507-6476
Catalogue number
CP-3001
Research area
Neurological & Cognitive
Date of manufacture
2025-07-07
Date of analysis
2025-07-15
Retest by
2028-06-29
Appearance
White lyophilised solid
Purity by RP-HPLC (area normalisation, 220 nm)
99.53%
Specification ≥ 99.0%
RELEASED
9/9 parameters within specification
Analytical results
| Test | Method | Specification | Result | Status |
|---|---|---|---|---|
| Appearance | Visual inspection | White to off-white lyophilised solid | White lyophilised solid | Pass |
| Identity | ESI-MS (positive ion) | [M+H]⁺ 814.93 ± 1.0 Da | 814.90 Da | Pass |
| Purity | RP-HPLC, 220 nm, C18 gradient | ≥ 99.0 % (area normalisation) | 99.53 % | Pass |
| Single largest impurity | RP-HPLC, 220 nm | ≤ 1.00 % | 0.300 % | Pass |
| Water content | Karl Fischer titration | ≤ 8.0 % | 4.6 % | Pass |
| Acetate content | Ion chromatography | ≤ 12.0 % | 5.0 % | Pass |
| Peptide content | Amino acid analysis | ≥ 70.0 % | 88.1 % | Pass |
| Bacterial endotoxin | LAL kinetic chromogenic | ≤ 0.50 EU/mg | 0.021 EU/mg | Pass |
| Solubility | Visual, per stated solvent | Freely soluble in water | Conforms | Pass |
Figure 1 · RP-HPLC chromatogram
C18, 4.6 × 250 mm, 5 µm. Water/acetonitrile gradient with 0.1% TFA over 20 minutes. Detection at 220 nm.
| Peak | Retention time | Area % | Assignment |
|---|---|---|---|
| 1 | 9.04 min | 0.027 | Acetylated analogue |
| 2 | 9.20 min | 0.109 | Diastereomer |
| 3 | 9.82 min | 99.530 | Semax |
| 4 | 10.72 min | 0.034 | Des-amido analogue |
| 5 | 11.96 min | 0.300 | Diastereomer |
Figure 2 · ESI mass spectrum
Positive ion electrospray. Observed charge states with theoretical comparison below.
Molecular formula
C37H51N9O10S
Theoretical [M+H]⁺
814.93 Da
Observed [M+H]⁺
814.90 Da
Sequence
Met-Glu-His-Phe-Pro-Gly-Pro
MEHFPGP